Interested in Ordering?

1. Email us for sequences and information at vectors@uiowa.edu. 

2. All plasmid requests must be placed online.  Click on the link at the top of the page to register for an iLabs Account. Log into the iLabs Website.

3. External Non University of Iowa Investigators must initiate the MTA form for distribution of pFBAAV plasmids owned and managed by NIH. Plasmids can be provided at no charge for viral production work intended for the University of Iowa VVC. 

4. University of Iowa Investigators: Order plasmids through the University of Iowa - Plasmids Request Form at the iLabs ordering site.

Non-Self Complementary Shuttle Plasmids

  • ITR to ITR cassette size is limited to ~4.7kb

G0202 pFBAAVmcsBgHpA

  • Cat: G0202
  • pFBAAV backbone, multiple cloning site, Bovine Growth Hormone polyadenylation signal

G0347 pFBAAVCMVmcspBgHpA

  • Cat: G0347
  • pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal

G0261 pFBAAVRSVmcsBgHpA

  • Cat: G0261
  • pFBAAV backbone, RSV (rocus sarcoma virus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal

G0345 pFBAAVCAGmcsBgHpA

  • Cat: G0345
  • pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, bovine growth hormone polyadenylation signal

G0746 pFBAAVmcswtIRESeGFPBgHpA

  • Cat: G0746
  • pFBAAV backbone, multiple cloning site, wild type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.

G0692 pFBAAVCMVmcswtIRESeGFPBgHpA

  • Cat: G0692
  • pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.

G0747 pFBAAVCAGmcswtIRESeGFPBgHpa

  • Cat: G0747
  • pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.

G1066 pFBAAVmcswtIRESmCherryBgHpA

  • Cat: G1066
  • pFBAAV backbone, multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.

G1068 pFBAAVCMVmcswtIRES
mCherryBgHpA

  • Cat: G1068
  • pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry, bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.

G1067 pFBAAVCAGmcswtIRES
mCherryBgHpA

  • Cat: G1067
  • pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry, bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.

G0358 pFBAAVmcsCMVhrGFPSV40pA

  • Cat: G0358
  • pFBAAV backbone, multiple cloning site, CMV (cytomegalovirus) promoter driving hrGFP (Humanized Renilla reniformis green fluorescent protein), SV40 polyadenylation signal.

G0619 pFBAAVmcsCMVeGFPSV40A

  • Cat: G0619
  • Extended mcs. pFBAAV backbone, multiple cloning site (more available sites), CMV (cytomegalovirus) promoter driving eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal

G0760 pFBAAVmU6mcsCMVeGFPSV40pA

  • Cat: G0760
  • pFBAAV backbone, mouse U6 promoter (RNA polymerase III), multiple cloning site designed for RNAi insertion, poly T termination sequence, CMV (cytomegalovirus) promoter driving eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal

G0774 pFBAAVmU6mcsCMVmCherrySV40pA

  • Cat: G0774
  • pFBAAV backbone, mouse U6 promoter (RNA polymerase III), multiple cloning site designed for RNAi insertion, poly T termination sequence, CMV (cytomegalovirus) promoter driving mCherry (red fluorescent protein), SV40 polyadenylation signal

G1006 pFBAAVmU6miSafeCMV
mCherrySV40pA

  • Cat: G1006
  • Scrambled control for testing use with cat: G0774.
  • Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.

G0784 pFBAAVmU6miSafeCMVeGFPSV40pA

  • Cat: G0784
  • Scrambled control for testing use with cat: G0760.
  • Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.

G1060 pFBAAVTREmcsBgHpACMVrtTA
wtIRESeGFPSV40pA

  • Cat: G1060
  • pFBAAV backbone, TRE (TET response element) promoter, multiple cloning site designed for RNAi insertion, bovine growth hormone polyadenylation signal, CMV (cytomegalovirus) promoter driving tetracycline-controlled transactivator (rtTA-M2), wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal. This plasmid was designed for inducible expression (TET-ON) of the miRNA cassette in the presence of the antibiotic tetracycline (doxycycline).

G1061 pFBAAVTREmiSafeBgHpACMVrtTA
wtIRESeGFPSV40pA

  • Cat: G1061
  • Scrambled control for testing use with cat: G1060.
  • Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.

G0013 pFBGR

  • Cat: G0013
  • pFBAAV backbone, CMV (cytomegalovirus) promoter and P10 insect promoter driving eGFP (enhanced green fluorescent protein) , SV40pA polyadenylation signal.

Self-Complementary Shuttle Plasmids

  • ITR to ITR cassette size is limited to ~2.2kb.

G0463 pscAAVmcsBgHpa

  • Cat: G0463
  • Self-complementary plasmid for use with the triple transfection system only. Multiple cloning site, bovine growth hormone polyadenylation signal. The backbone is based on self-complementary AAV vectors kindly provided by Brian Kasper.

G0605 pscAAVmCMVmcsBgHpA

  • Cat: G0605
  • Self-complementary plasmid for use with the triple transfection system only. Modified CMV (cytomegalovirus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal. The backbone is based on self-complementary AAV vectors kindly provided by Brian Kasper.