Interested in Ordering?

Please view our ordering information for custom constructs to learn how to register for an account, place orders for in-stock vectors and make payment through our online system.

Pricing

New Custom Vectors from recombination: $4,045 for the yield from 40 plates with a minimum of 2 x 1ml standard titer. New Custom Vectors from amplified particles: $3,470 for the yield from 40 plates with a minimum of  2 x 1ml standard titer. Re-order Custom Vectors from amplified particles: $3,034 for the yield from 40 plates. Contact vectors@uiowa.edu for high titer or small aliquot pricing. Pricing listed is for external academic non-profit customers. Please contact us for a specific quote.

Description

Viral vector construction is performed using the RapAdTM System developed by the University of Iowa VVC (For description, refer to from the article "A simple method for the rapid generation of recombinant adenovirus vectors" published in Gene Therapy 7:1034-1038, 2000). Adenovirus vectors prepared in the core are E1 and E3 deleted. They have a total E1a deletion (*m.u 1.4 to 4.5) plus a partial E1b deletion (*m.u. 4.7 to 9.2). These deletions are what make the vector replication deficient. They also have a partial E3 deletion, 720bp for the sub360 backbone, a 1.6 kb deletion for the dl309 backbone and a 3.1 kb deletion for the total E3 deleted backbone.
*m.u = Map units

Characteristics

  • Episomal gene expression.
  • Infects dividing and non-dividing cells.
  • Transient high-level protein expression.
  • Accommodates inserts of up to 7.5kb. Larger inserts can be added, provided that an equivalent part of the viral genome has been properly deleted.
  • High viral titer can be produced: 1 x 1010 pfu/ml to 1 x 1011 pfu/ml.

Disadvantages and Adverse Effects

Elicits host immune response, thus depleting the number of transduced cells over time in-vivo.

Short-term expression of the transgene due to lack of integration into the host genome.

Shuttle Vectors

Please see the shuttle plasmids page for a complete list of available shuttle vectors.

References

RapAdTM System: Anderson RD, Haskell RE, Xia H, Roessler BJ, Davidson BL. “A simple method for the rapid generation of recombinant adenovirus vectors”. Gene Ther. 2000 Jun;7(12):1034-8.

A195 Buffer: Evans RK, Nawrocki DK, Isopi LA, Williams DM, Casimiro DR, Chin S, Chen M, Zhu DM, Shiver JW, Volkin DB. Development of stable liquid formulations for adenovirus-based vaccines. J Pharm Sci. 2004 Oct;93(10):2458-75.

Plasmid Quality Control upon Submission

Every effort is made to deliver a high titer, high quality viral vector. We require gene information, a sequence, map, and a full plasmid sequencing upon submission of a new project. High quality supercoiled, endotoxin-free, shuttle plasmid DNA is critical to the success of the initial transfection and recombination. We are able to outsource the plasmid prep service to Genscript by quote. We recommend functionally test the plasmid before submission. 

Despite best efforts, some genes of interest may confer cellular toxicity that results in lower vector titers. We will notify investigators of progress and discuss the next steps if problems are noted.